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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Rifampicin Induces Gene, Protein, and Activity of P-Glycoprotein (ABCB1) in Human Precision-Cut Intestinal Slices
doi: 10.3389/fphar.2021.684156
Figure Lengend Snippet: ABCB1 , CYP3A4 , PXR , and RXRA gene expression in hPCIS determined by qRT-PCR. Fold changes in ABCB1 and CYP3A4 mRNA levels in hPCISs incubated with RIF (30 µM) for 4, 8, 12, 16, 20, 24, and 48 h (A) , relative to expression in RIF-free controls, showing that RIF significantly increased mRNA levels of both genes in samples from all donors at all tested time points (one-sample t -tests, three slices from one donor: *, p < 0.05; **, p < 0.01; ***, p < 0.001). PXR (B) and RXRA (C) were demonstrated to be expressed in hPCISs incubated with RIF (30 µM) at all tested time points. Amounts of PXR and RXRA transcripts are presented in arbitrary units (a.u.), calculated as 2 −∆Ct × 10 6 . (D) The matrix of Pearson’s coefficients of correlation was used to assess associations among all targeted transcripts in RIF-treated hPCISs (positive in blue and negative in red, with color intensity proportional to the magnitude of the coefficients). Asterisks indicate significant differences: *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Article Snippet:
Techniques: Gene Expression, Quantitative RT-PCR, Incubation, Expressing